NB100-214

antibody from Novus Biologicals

Targeting: BLM BS, RECQ2, RECQL3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-214

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-214, RRID:AB_10001628

Product name

Rabbit Polyclonal Blooms Syndrome Protein Blm Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Increased error-free DNA repair gene expression through reprogramming in human iPS cells.

Yoshimura Y
Regenerative therapy 2019 Dec;11:101-105

---

Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.

Nicholls TJ, Nadalutti CA, Motori E, Sommerville EW, Gorman GS, Basu S, Hoberg E, Turnbull DM, Chinnery PF, Larsson NG, Larsson E, Falkenberg M, Taylor RW, Griffith JD, Gustafsson CM
Molecular cell 2018 Jan 4;69(1):9-23.e6

---

The WRN and MUS81 proteins limit cell death and genome instability following oncogene activation.

Murfuni I, Nicolai S, Baldari S, Crescenzi M, Bignami M, Franchitto A, Pichierri P
Oncogene 2013 Jan 31;32(5):610-20

---

BLM helicase stimulates the ATPase and chromatin-remodeling activities of RAD54.

Srivastava V, Modi P, Tripathi V, Mudgal R, De S, Sengupta S
Journal of cell science 2009 Sep 1;122(Pt 17):3093-103

---

HYD1-induced increase in reactive oxygen species leads to autophagy and necrotic cell death in multiple myeloma cells.

Nair RR, Emmons MF, Cress AE, Argilagos RF, Lam K, Kerr WT, Wang HG, Dalton WS, Hazlehurst LA
Molecular cancer therapeutics 2009 Aug;8(8):2441-51

---

BLM helicase-dependent and -independent roles of 53BP1 during replication stress-mediated homologous recombination.

Tripathi V, Nagarjuna T, Sengupta S
The Journal of cell biology 2007 Jul 2;178(1):9-14

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 0.1 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Whole cell lysate from 293T cells (35 ug).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (50 ug) from TCMK-1 and BIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (15 ug) from HEK293T, K-562, Jurkat, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.