Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- 19762-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR50 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of anti-GPR50 (19762-1-AP, 1:2000) by Dr. Qian Li, University of Edinburgh. U1: SH-SY5Y cells, untransfected, 10ug; G1: HEK293 cells, with transfected GPR50, 1ug; G2: HEK293 cells, with transfected GPR50, 10ug; U2: HEK293 cells, untransfected, with endogenous GPR50, 10ug; U3: HEK293 cells, untransfected, with endogenous GPR50, 20ug.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of anti-GPR50 (19762-1-AP, 1:500) with SH-SY5Y cells transfected with GPR50 by Dr. Qian Li, University of Edinburgh.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of anti-GPR50 (19762-1-AP, 1:50) with mouse brain tissue slide by Dr. Qian Li, University of Edinburgh.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 19762-1-AP ( GPR50 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 19762-1-AP ( GPR50 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 K-562 cells were stained with 0.2ug GPR50 antibody (19762-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.