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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- 21514-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR50 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LFAVCWCPI NVLTVLVAVS PKEMAGKIPN WLYLAAYFIA YFNSCLNAVI YGLLNENFRR EYWTIFHAMR HPIIFFSGLI SDIREMQEAR TLARARAHAR DQAREQDRAH ACPAVEETPM NVRNVPLPGD AAAGHPDRAS GHPKPHSRSS SAYRKSASTH HKSVFSHSKA ASGHLKPVSG HSKPASGHPK SATVYPKPAS VHFKADSVHF KGDSVHFKPD SVHFKPASSN PKPITGHHVS AGSHSKSAFS AATSHPKPTT GHIKPATSHA EPTTADYPKP ATTSHPKPTA ADNPELSASH CPEIPAIAHP VSDDSDLPES ASSPAAGPTK PAASQLESDT IADLPDPTVV TTSTNDYHDV VVVDVEDDPD EMAV (245-617 aa encoded by B C103696)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of GPR50 antibody (21514-1-AP, 1:5,000) by Dr. Qian Li, University of Edinburgh. U1: Lysates from SH-SY5Y cells, untransfected, no endogenous GPR50, negative control,15ug; G1: Lysates from HEK293 cells, with transfected GPR50, positive control, 2ug; G2: Lysates from HEK293 cells, with transfected GPR50, 10ug; U2: Lysates from HEK293 cells, untransfected, with endogenous GPR50, 10ug; U3: Lysates from HEK293 cells, untransfected, with endogenous GPR50, 10ug. Optimal dilution: 1 in 5000, Signal detection: ECL, Pierce; Exposure time: 10min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21514-1-AP (GPR50 antibody) at dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of GPR50 antibody (21514-1-AP, 1:1,000) with SH-SY5Y cells transfected with GPR50 by Dr. Qian Li, University of Edinburgh.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of GPR50 antibody (21514-1-AP, 1:1,000) with SH-SY5Y cells transfected with GPR50 by Dr. Qian Li, University of Edinburgh.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of GPR50 antibody (21514-1-AP, 1:50) with mouse hippocampus by Dr. Qian Li, University of Edinburgh.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 K-562 cells were stained with 0.2ug GPR50 antibody (21514-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
