200-301-G48
antibody from Rockland Immunochemicals, Inc.
Targeting: SNAP25
bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-301-G48 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-G48, RRID:AB_2611246
- Product name
- Anti-SNAP-25 (MOUSE) Monoclonal Antibody - 200-301-G48
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Anti-SNAP-25 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with SNAP-25 from human, Mouse, Rat, Cat, and Pig based on 100% homology with the immunizing sequence. Cross-reactivity with SNAP-25 from other sources has not been determined. Neurobiology research.
- Reactivity
- Human, Mouse, Rat, Feline, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- SP-12
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
- Storage
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Handling
- Dry Ice
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of mouse anti-SNAP25 antibody. Lane 1: Human Cell line mix. Primary antibody: SNAP25 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-mouse IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4°C. Predicted/Observed size:23.3 kDa/29kD. Other band(s): none.
- Validation comment
- Western Blot