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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
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- Product number
- 24935-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEC61alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: TIVWKAFSP TTINTGRGTE FEGAVIALFH LLATRTDKVR ALREAFYRQN LPNLMNLIAT VFVFAVVIYF QGFRVDLPIK SARYRGQYSS YPIKLFYTSN IPIILQSALV SNLYVISQML SVRFSGNFLV NLLGQWADVS GGGPARSYPV GGLCYYLSPP ESMGAIFEDP VHVVVYIIFM LGSCAFFSKT WIEVSGSSAK DVAKQLKEQQ MVMRGHRDTS MVHELNSARA (190-418 aa encoded by BC026179)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.35 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP ( SEC61alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 24935-1-AP (SEC61alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 24935-1-AP (SEC61alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
