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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-30897 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFIX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG.
- Concentration
- 1.23 mg/mL
Submitted references Mouse Retinal Cell Atlas: Molecular Identification of over Sixty Amacrine Cell Types.
Yan W, Laboulaye MA, Tran NM, Whitney IE, Benhar I, Sanes JR
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Jul 1;40(27):5177-5195
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Jul 1;40(27):5177-5195
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFIX using 30 µg of U87-MG lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a NFIX polyclonal antibody (Product # PA5-30897) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFIX was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFIX Polyclonal Antibody (Product # PA5-30897) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NFIX was performed in paraffin-embedded mouse hippocampus tissue using NFIX Polyclonal Antibody (Product # PA5-30897) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NFIX was performed in paraffin-embedded mouse hippocampus tissue using NFIX Polyclonal Antibody (Product # PA5-30897) at a dilution of 1:500.
