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LearnAF6254
antibody from R&D Systems
Targeting: HCFC1
CFF, HCF-1, HCF1, HFC1, MGC70925, MRX3, PPP1R89, VCAF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF6254 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Host Cell Factor 1/HCFC1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Host Cell Factor 1/HCFC1 in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P51610- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Host Cell Factor 1/HCFC1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Host Cell Factor 1/HCFC1 at approximately 300 kDa (as indicated) and proteolytic fragments of Host Cell Factor 1/HCFC1 at approximately 100 to 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Host Cell Factor 1/HCFC1 in HeLa Human Cell Line. Host Cell Factor 1/HCFC1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Host Cell Factor 1/HCFC1 in Human Colon Cancer Tissue. Host Cell Factor 1/HCFC1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
