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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
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- Product number
- 48-5200 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-NMDAR2B (Tyr1252) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Description: The monoclonal antibody DM1a recognizes the 50 kDa cytoskeletal protein alpha tubulin in a variety of species (human, mouse, rat, monkey, dog, pig, bovine, goat, hamster, guinea pig, kangaroo, amphibians, sea urchin, yeast and tobacco plants). Tubulin, the major component of microtubules, is a dimeric protein consisting of an alpha and beta subunit. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation resulting in assembly (polymerization) or disassembly (depolymerization). The dynamic nature of microtubules is most evident in the mitotic apparatus. The DM1a antibody recognizes the C-terminal end of the alpha tubulin isoform (amino acids 426-430).
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Synaptic proximity enables NMDAR signalling to promote brain metastasis.
GKAP Acts as a Genetic Modulator of NMDAR Signaling to Govern Invasive Tumor Growth.
Zeng Q, Michael IP, Zhang P, Saghafinia S, Knott G, Jiao W, McCabe BD, Galván JA, Robinson HPC, Zlobec I, Ciriello G, Hanahan D
Nature 2019 Sep;573(7775):526-531
Nature 2019 Sep;573(7775):526-531
GKAP Acts as a Genetic Modulator of NMDAR Signaling to Govern Invasive Tumor Growth.
Li L, Zeng Q, Bhutkar A, Galván JA, Karamitopoulou E, Noordermeer D, Peng MW, Piersigilli A, Perren A, Zlobec I, Robinson H, Iruela-Arispe ML, Hanahan D
Cancer cell 2018 Apr 9;33(4):736-751.e5
Cancer cell 2018 Apr 9;33(4):736-751.e5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat hippocampal lysate using rabbit anti-NMDA NR2B (pY1252) antibody (Product # 48-5200). After transfer, the membrane was left untreated (lane 1-control) or treated with lambda phosphatase (1200 units for 30 min) before being exposed to the antibody (lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat hippocampal lysate using rabbit anti-NMDA NR2B (pY1252) antibody (Product # 48-5200). After transfer, the membrane was left untreated (lane 1-control) or treated with lambda phosphatase (1200 units for 30 min) before being exposed to the antibody (lane 2).
