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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
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- Product number
- NBP1-49924 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49924, RRID:AB_10012114
- Product name
- Rabbit Polyclonal Gigaxonin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Kelch Domain of Gigaxonin Interacts with Intermediate Filament Proteins Affected in Giant Axonal Neuropathy.
Johnson-Kerner BL, Garcia Diaz A, Ekins S, Wichterle H
PloS one 2015;10(10):e0140157
PloS one 2015;10(10):e0140157
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. Endogenous VIM pulls down endogenous GIG. Indicated lysates were subjected to immunoprecipitation with either VIM antibody or control IgG (used as the mock condition throughout), and the membrane was probed sequentially for endogenous GIG and then VIM (not shown). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Gigaxonin antibody NBP1-49924 used for IP at 3 ug/mg lysate. Gigaxonin was also immunoprecipitated by rabbit anti-Gigaxonin antibody NBP1-49923, which recognizes an upstream epitope. For blotting immunoprecipitated Gigaxonin, NBP1-49924 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. GIG pulls down overexpressed PRPH in a Kelch-dependent manner. Cell lysates were subjected to immunoprecipitation with anti-Flag beads or IgG, and the membrane was probed sequentially for PRPH then GIG (not shown). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY licence.
