Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-22212 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal PI 3-Kinase p85 alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Protease-activated receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway.
Du C, Zhang T, Xiao X, Shi Y, Duan H, Ren Y
The Biochemical journal 2017 Aug 2;474(16):2733-2747
The Biochemical journal 2017 Aug 2;474(16):2733-2747
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis using PI 3 Kinase p85 alpha mAb against human PI 3 Kinase p85 alpha recombinant protein. (Expected MW is 53.4 kDa)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of rectum cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of cervical cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of NIH3T3 cells using PI 3 Kinase p85 alpha mouse mAb (green) and negative control (red).