AM7628b

antibody from Abgent

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM7628b

Provider

Abgent

Proper citation

Abgent Cat#AM7628b, RRID:AB_1208633

Product name

EGFR Antibody

Antibody type

Monoclonal

Antigen

Recombinant Protein

Description

Purified Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of lysate from A431 cell line, using EGFR Antibody(Cat. #AM7628b). AM7628b was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug."

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human breast carcinoma with EGFR Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using EGFR Monoclonal Antibody(bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50