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Western blot
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX100448 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100448, RRID:AB_1950176
- Product name
- EGFR antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Quercetin Reverses Rat Liver Preneoplastic Lesions Induced by Chemical Carcinogenesis.
Salvianolic acid B maintained stem cell pluripotency and increased proliferation rate by activating Jak2-Stat3 combined with EGFR-Erk1/2 pathways.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Quercetin Reverses Rat Liver Preneoplastic Lesions Induced by Chemical Carcinogenesis.
Carrasco-Torres G, Monroy-Ramírez HC, Martínez-Guerra AA, Baltiérrez-Hoyos R, Romero-Tlalolini MLÁ, Villa-Treviño S, Sánchez-Chino X, Vásquez-Garzón VR
Oxidative medicine and cellular longevity 2017;2017:4674918
Oxidative medicine and cellular longevity 2017;2017:4674918
Salvianolic acid B maintained stem cell pluripotency and increased proliferation rate by activating Jak2-Stat3 combined with EGFR-Erk1/2 pathways.
Liu CH, Shyu WC, Fu RH, Huang SJ, Chang CH, Huang YC, Chen SY, Lin SZ, Liu SP
Cell transplantation 2014;23(4-5):657-68
Cell transplantation 2014;23(4-5):657-68
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) A431 whole cell extracts (15 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 5% SDS PAGE GTX100448 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) A431 whole cell extracts (15 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using EGFR(GTX100448) antibody (Green) at 1:1000 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EGFR antibody [C2C3], C-term immunoprecipitates EGFR protein in IP experiments.IP samples: A431 whole cell extractA. 40 £gg A431 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of EGFR protein by 4 £gg EGFR antibody [C2C3], C-term (GTX100448)5 % SDS-PAGEThe immunoprecipitated EGFR protein was detected by EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer . EGFR antibody [C2C3], C-term (GTX100448) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. EGFR antibody [C2C3], C-term (GTX100448) dilution: 1:500.
