102-PA06S

antibody from ReliaTech GmbH

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

102-PA06S

Provider

ReliaTech GmbH

Product name

Anti-human EGFR

Antibody type

Polyclonal

Antigen

Other

Description

antibody Protein-A purified from serum

Reactivity

Human

Host

Rabbit

Antigen sequence

LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCE
VVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALN
TVERIPLENLQIIRGNMYYENSYALAVLSNYDANK
TGLKELPMRNLQEILHGAVRFSNNPALCNVESIQW
RDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSC
WGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHN
QCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLML
YNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDH
GSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNG
IGIGEFKDSLSINATNIKHFKNCTSISGDLHILPV
AFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQA
WPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLN
ITSLGLRSLKEISDGDVIISGNKNLCYANTINWKK
LFGTSGQKTKIISNRGENSCKATGQVCHALCSPEG
CWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREF
VENSECIQCHPECLPQAMNITCTGRGPDNCIQCAH
YIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHL
CHPNCTYGCTGPGLEGCPTNGPKIPS


Vial size

100 µg

Storage

The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Western analysis of recombinant human soluble EGFR using a polyclonal rabbit anti-human EGFR [Cat# 102-PA06].

Sample type

Purified recombinant protein

ELISA

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Binding of anti-human EGFR [Cat# 102-PA06] to recombinant human soluble EGFR [Cat# S01-040] in a functional ELISA. sEGFR was coated starting with 122 pg/ml to 2000 ng/ml (100µl/well). Detection was performed using a polyclonal rabbit anti-human EGFR (1 µg/ml, 100 µl/well) and a goat anti-rabbit Biotin conjugated secondary antibody.

Sample type

Purified recombinant protein

Immunocytochemistry

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Immunofluorescence staining of human EGFR in the human epidermoid carcinoma cell line A431 with a polyclonal rabbit anti-human EGFR antibody [Cat# 102-PA06]. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].

Sample type

A431 cells