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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- ARG54163 - Provider product page
- Provider
- Arigo
- Product name
- anti-EGFR antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human EGFR protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3F12-1H7-A10
- Vial size
- 100 µl
- Concentration
- 1.1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: EGFR in HCC827, A549 and MDA-MB-468 cell lysates stained with EGFR mouse mAb (diluted in 1: 1000). Predicted band size: 134 Kda. Observed band size: 175KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with ARG54163 EGF Receptor antibody [3F12-1H7-A10] (diluted in 1:200).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: MDA-MB-468 cells fixed with 4% Paraformaldehyde and stained with ARG54163 EGF Receptor antibody [3F12-1H7-A10] (diluted in 1:200).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: HeLa cell lysates immunoprecipitated and stained by ARG54163 EGF Receptor antibody [3F12-1H7-A10]
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Lung carcinoma stained with EGF Receptor (3F12) mouse mAb (diluted in 1/800) at the Roche Benchmark XT system.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Colorectal cancer stained with EGF Receptor (3F12) mouse mAb (diluted in 1/800). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6. 0).
