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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Protein array [1]
- Other assay [1]
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- Product number
- V2485 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EGFR Antibody
- Antibody type
- Monoclonal
- Antigen
- Human EGF Receptor purified from A431 cells was used as the immunogen for the EGFR antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 31G7
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the EGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Polymorphisms in poly (ADP-ribose) polymerase-1 (PARP1) promoter and 3' untranslated region and their association with PARP1 expression in breast cancer patients.
A Phase II Study of Sorafenib Combined With Cetuximab in EGFR-Expressing, KRAS-Mutated Metastatic Colorectal Cancer.
Transformation to large cell neuroendocrine carcinoma as acquired resistance mechanism of EGFR tyrosine kinase inhibitor.
Dual gain of HER2 and EGFR gene copy numbers impacts the prognosis of carcinoma ex pleomorphic adenoma.
Phase II study of gefitinib in patients with advanced salivary gland cancers.
Positive Interplay Between CD3+ T-lymphocytes and Concurrent COX-2/EGFR Expression in Canine Malignant Mammary Tumors.
Adult classical glioblastoma with a BRAF V600E mutation.
The Prognostic Significance of c-MET and EGFR Overexpression in Resected Gastric Adenocarcinomas.
Epidermal Growth Factor Receptor Expression May Correlate with Survival Through Clinical and Radiological Features of Aggressiveness in Glioblastoma Treated with Radiochemotherapy.
Epidermal growth factor receptor expression in canine transitional cell carcinoma.
Breast cancer in very young women: Clinicopathological study of 149 patients ≤25 years old.
Zhai L, Li S, Li H, Zheng Y, Lang R, Fan Y, Gu F, Guo X, Zhang X, Fu L
International journal of clinical and experimental pathology 2015;8(6):7059-71
International journal of clinical and experimental pathology 2015;8(6):7059-71
A Phase II Study of Sorafenib Combined With Cetuximab in EGFR-Expressing, KRAS-Mutated Metastatic Colorectal Cancer.
Do K, Cao L, Kang Z, Turkbey B, Lindenberg ML, Larkins E, Holkova B, Steinberg SM, Raffeld M, Peer CJ, Figg WD, Eugeni M, Jacobs P, Choyke P, Wright JJ, Doroshow JH, Kummar S
Clinical colorectal cancer 2015 Sep;14(3):154-61
Clinical colorectal cancer 2015 Sep;14(3):154-61
Transformation to large cell neuroendocrine carcinoma as acquired resistance mechanism of EGFR tyrosine kinase inhibitor.
Kogo M, Shimizu R, Uehara K, Takahashi Y, Kokubo M, Imai Y, Tomii K
Lung cancer (Amsterdam, Netherlands) 2015 Nov;90(2):364-8
Lung cancer (Amsterdam, Netherlands) 2015 Nov;90(2):364-8
Dual gain of HER2 and EGFR gene copy numbers impacts the prognosis of carcinoma ex pleomorphic adenoma.
Nishijima T, Yamamoto H, Nakano T, Nakashima T, Taguchi K, Masuda M, Motoshita J, Komune S, Oda Y
Human pathology 2015 Nov;46(11):1730-43
Human pathology 2015 Nov;46(11):1730-43
Phase II study of gefitinib in patients with advanced salivary gland cancers.
Jakob JA, Kies MS, Glisson BS, Kupferman ME, Liu DD, Lee JJ, El-Naggar AK, Gonzalez-Angulo AM, Blumenschein GR Jr
Head & neck 2015 May;37(5):644-9
Head & neck 2015 May;37(5):644-9
Positive Interplay Between CD3+ T-lymphocytes and Concurrent COX-2/EGFR Expression in Canine Malignant Mammary Tumors.
Carvalho MI, Pires I, Prada J, Ferreira AF, Queiroga FL
Anticancer research 2015 May;35(5):2915-20
Anticancer research 2015 May;35(5):2915-20
Adult classical glioblastoma with a BRAF V600E mutation.
Takahashi Y, Akahane T, Sawada T, Ikeda H, Tempaku A, Yamauchi S, Nishihara H, Tanaka S, Nitta K, Ide W, Hashimoto I, Kamada H
World journal of surgical oncology 2015 Mar 11;13:100
World journal of surgical oncology 2015 Mar 11;13:100
The Prognostic Significance of c-MET and EGFR Overexpression in Resected Gastric Adenocarcinomas.
Paliga A, Marginean H, Tessier-Cloutier B, Purgina B, Jonker D, Marginean EC
American journal of clinical oncology 2015 Jun 29;
American journal of clinical oncology 2015 Jun 29;
Epidermal Growth Factor Receptor Expression May Correlate with Survival Through Clinical and Radiological Features of Aggressiveness in Glioblastoma Treated with Radiochemotherapy.
Tini P, Cerase A, Cevenini G, Carbone SF, Miracco C, Pirtoli L
Anticancer research 2015 Jul;35(7):4117-24
Anticancer research 2015 Jul;35(7):4117-24
Epidermal growth factor receptor expression in canine transitional cell carcinoma.
Hanazono K, Fukumoto S, Kawamura Y, Endo Y, Kadosawa T, Iwano H, Uchide T
The Journal of veterinary medical science / the Japanese Society of Veterinary Science 2015 Jan;77(1):1-6
The Journal of veterinary medical science / the Japanese Society of Veterinary Science 2015 Jan;77(1):1-6
Breast cancer in very young women: Clinicopathological study of 149 patients ≤25 years old.
de Deus Moura R, Carvalho FM, Bacchi CE
Breast (Edinburgh, Scotland) 2015 Aug;24(4):461-7
Breast (Edinburgh, Scotland) 2015 Aug;24(4):461-7
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human placenta stained with EGFR antibody (31G7).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using EGFR antibody (clone 31G7). These results demonstrate the foremost specificity of the 31G7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free EGFR Antibody (clone 31G7). Confirmation of Integrity and Purity of the Antibody.
