Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM00036PU-N - Provider product page
- Provider
- OriGene
- Product name
- EGFR pSer1047 (incl. pos. control) mouse monoclonal antibody, clone 1H9, Purified
- Antibody type
- Monoclonal
- Description
- EGFR pSer1047 (incl. pos. control) mouse monoclonal antibody, clone 1H9, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- EGFR
- Isotype
- IgG
- Antibody clone number
- 1H9
- Vial size
- 100 µg
Submitted references Differential regulation of epidermal growth factor receptor by hydrogen peroxide and flagellin in cultured lung alveolar epithelial cells.
Nishi H, Maeda N, Izumi S, Higa-Nakamine S, Toku S, Kakinohana M, Sugahara K, Yamamoto H
European journal of pharmacology 2015 Feb 5;748:133-42
European journal of pharmacology 2015 Feb 5;748:133-42
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- EGFR Transactivation: Serum starved HepG2 cells were treated for 15min as indicated . Whole cell lysates were separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab EGFR-1H9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: PMA Lane 3: Forskolin Lane 4: LPA Lane 5: Sorbit Lane 6; Anisomycin Lane 7: Ionomycin Lane 8: Taxol
- Validation comment
- WB
