PAB10223

antibody from Abnova Corporation

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10223

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10223, RRID:AB_1674726

Product name

EGFR polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of EGFR.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The EGF receptor provides an essential survival signal for SOS-dependent skin tumor development.

Sibilia M, Fleischmann A, Behrens A, Stingl L, Carroll J, Watt FM, Schlessinger J, Wagner EF
Cell 2000 Jul 21;102(2):211-20

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Role of epidermal growth factor receptor and STAT-3 activation in autonomous proliferation of SUM-102PT human breast cancer cells.

Sartor CI, Dziubinski ML, Yu CL, Jove R, Ethier SP
Cancer research 1997 Mar 1;57(5):978-87

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Occurrence of epidermal growth factor receptors in benign and malignant ovarian tumors and normal ovarian tissues: an immunohistochemical study.

Henzen-Logmans SC, van der Burg ME, Foekens JA, Berns PM, Brussée R, Fieret JH, Klijn JG, Chadha S, Rodenburg CJ
Journal of cancer research and clinical oncology 1992;118(4):303-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using EGFR polyclonal antibody (Cat # PAB10223) shows detection of a band at ~170 KDa corresponding to human EGFR present in unstimulated (Lane 1) and EGF (50 ng/mL for 15 min) stimulated (Lane 2) A-431 whole cell lysates (arrowhead).Approximately 30 ug of lysate was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking, the membrane was probed with the primary antibody diluted to 1:1,000.Reaction occurred overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Combined immunoprecipitation and immunoblot using EGFR polyclonal antibody (Cat # PAB10223).Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15' at 100 ng/mL and without (-) the addition of EGF.The combination of immunoprecipitation and immunoblotting was performed using the EGFR polyclonal antibody (Cat # PAB10223) for immunoprecipitation (10 uL) followed by immunoblot detection using an anti-phosphotyrosine antibody (Panel A).This was repeated in reverse order using a 1:2000 dilution of EGFR polyclonal antibody for immunoblot (Panel B).Visualization occurred using an ECL system.Film exposure was approximately 1'.

Validation comment

Immunoprecipitation

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining with EGFR polyclonal antibody (Cat # PAB10223) was diluted to 2.5 ug/mL detect EGFR in human ovarian cancer tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)