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LearnSTJ99228
antibody from St John's Laboratory
Targeting: EGFR
ERBB, ERBB1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- STJ99228 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-EGFR antibody [3F12-1H7-A10] (STJ99228)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Epidermal Growth Factor Receptor is suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation research applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of EGFR in HCC827, A549 and MDA-MB-468 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Colorectal cancer using EGF Receptor (3F12) mouse mAb (1/800 dilution). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Lung carcinoma using EGF Receptor (3F12) mouse mAb (1/800 dilution) at the Roche Benchmark XT system.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
