933901

antibody from BioLegend

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

933901

Provider

BioLegend

Product name

Purified anti-EGFR

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

A19002A

Vial size

25 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Whole cell extracts (15 µg protein) from MOLT-4 (negative control), HeLa, PC-3, HaCaT, and A-431 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-EGFR antibody (clone A19002A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker.

Conjugate

Unconjugated