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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunohistochemistry [1]
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- Product number
- 100-401-149 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-149, RRID:AB_2293276
- Product name
- Anti-EGFR (RABBIT) Antibody - 100-401-149
- Antibody type
- Polyclonal
- Vial size
- 250 µl
Submitted references Control of plasma membrane lipid homeostasis by the extended synaptotagmins.
EGFR-targeted Chimeras of Pseudomonas ToxA released into the extracellular milieu by attenuated Salmonella selectively kill tumor cells.
Sensitivity of human granulosa cell tumor cells to epidermal growth factor receptor inhibition.
Evaluation of the cytotoxic effects of ophthalmic solutions containing benzalkonium chloride on corneal epithelium using an organotypic 3-D model.
Downregulation of EGF receptor signaling in pancreatic islets causes diabetes due to impaired postnatal beta-cell growth.
Saheki Y, Bian X, Schauder CM, Sawaki Y, Surma MA, Klose C, Pincet F, Reinisch KM, De Camilli P
Nature cell biology 2016 May;18(5):504-15
Nature cell biology 2016 May;18(5):504-15
EGFR-targeted Chimeras of Pseudomonas ToxA released into the extracellular milieu by attenuated Salmonella selectively kill tumor cells.
Quintero D, Carrafa J, Vincent L, Bermudes D
Biotechnology and bioengineering 2016 Dec;113(12):2698-2711
Biotechnology and bioengineering 2016 Dec;113(12):2698-2711
Sensitivity of human granulosa cell tumor cells to epidermal growth factor receptor inhibition.
Andersson N, Anttonen M, Färkkilä A, Pihlajoki M, Bützow R, Unkila-Kallio L, Heikinheimo M
Journal of molecular endocrinology 2014 Apr;52(2):223-34
Journal of molecular endocrinology 2014 Apr;52(2):223-34
Evaluation of the cytotoxic effects of ophthalmic solutions containing benzalkonium chloride on corneal epithelium using an organotypic 3-D model.
Khoh-Reiter S, Jessen BA
BMC ophthalmology 2009 Jul 28;9:5
BMC ophthalmology 2009 Jul 28;9:5
Downregulation of EGF receptor signaling in pancreatic islets causes diabetes due to impaired postnatal beta-cell growth.
Miettinen PJ, Ustinov J, Ormio P, Gao R, Palgi J, Hakonen E, Juntti-Berggren L, Berggren PO, Otonkoski T
Diabetes 2006 Dec;55(12):3299-308
Diabetes 2006 Dec;55(12):3299-308
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
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- Experimental details
- Combined immunoprecipitation and western blot using anti-EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15' at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 µl) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1. Other detection systems will yield similar results.
- Validation comment
- Immunohistochemistry
