Ab101163

antibody from Aladdin Scientific Corporation

Targeting: EGFR ERBB, ERBB1

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

Ab101163

Provider

Aladdin Scientific Corporation

Product name

EGFR Mouse mAb

Antibody type

Monoclonal

Description

Mouse anti Human EGFR Antibody, Monoclonal(C4), could be used for WB, IHC and so on .Application:WB: 0.01-2 µg/mLIHC: 5-20 µg/mLProtein FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.=Isoform 2 may act as an antagonist of EGF action.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

AA 888-1210


Epitope

reacts with an epitope located in the C terminal region of EGF receptor.

Isotype

IgG

Antibody clone number

C4

Vial size

100μl,10μl,1ml,20μl,50μl

Concentration

Lot by Lot

Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.