Antibody data
- Antibody Data
- Antigen structure
- References [64]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [5]
- Flow cytometry [1]
- Other assay [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-13319 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EGFR Monoclonal Antibody (199.12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-13319 targets Epidermal Growth Factor Receptor in IP, ICC/IF and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 199.12
- Concentration
- 0.2 mg/mL
Submitted references SCAMP3 Regulates EGFR and Promotes Proliferation and Migration of Triple-Negative Breast Cancer Cells through the Modulation of AKT, ERK, and STAT3 Signaling Pathways.
EGFR-targeted fluorescent imaging using the da Vinci® Firefly™ camera for gallbladder cancer.
EGFR Transgene Stimulates Spontaneous Formation of MCF7 Breast Cancer Cells Spheroids with Partly Loss of HER3 Receptor.
Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells.
Receptor-based mechanism of relative sensing and cell memory in mammalian signaling networks.
Maximum Entropy Framework for Predictive Inference of Cell Population Heterogeneity and Responses in Signaling Networks.
Breast Cancer Cell Invasion into a Three Dimensional Tumor-Stroma Microenvironment.
Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis.
EGFR Overexpressed in Colonic Neoplasia Can be Detected on Wide-Field Endoscopic Imaging.
Surfactant protein D suppresses lung cancer progression by downregulation of epidermal growth factor signaling.
mMAPS: a flow-proteometric technique to analyze protein-protein interactions in individual signaling complexes.
Internalization mechanisms of the epidermal growth factor receptor after activation with different ligands.
Propranolol restricts the mobility of single EGF-receptors on the cell surface before their internalization.
Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays.
Monitoring the size and lateral dynamics of ErbB1 enriched membrane domains through live cell plasmon coupling microscopy.
A Miniaturized Ligand Binding Assay for EGFR.
Differential roles of Grb2 and AP-2 in p38 MAPK- and EGF-induced EGFR internalization.
Superior antitumoral activity of dimerized targeted single-chain TRAIL fusion proteins under retention of tumor selectivity.
Novel membrane-associated androgen receptor splice variant potentiates proliferative and survival responses in prostate cancer cells.
Pnck induces ligand-independent EGFR degradation by probable perturbation of the Hsp90 chaperone complex.
High speed digital protein interaction analysis using microfluidic single molecule detection system.
IL-24 is expressed during wound repair and inhibits TGFalpha-induced migration and proliferation of keratinocytes.
EGFR and EGFRvIII interact with PUMA to inhibit mitochondrial translocalization of PUMA and PUMA-mediated apoptosis independent of EGFR kinase activity.
Spatial control of EGF receptor activation by reversible dimerization on living cells.
EGFRvIII-induced estrogen-independence, tamoxifen-resistance phenotype correlates with PgR expression and modulation of apoptotic molecules in breast cancer.
Nuclear EGFR is required for cisplatin resistance and DNA repair.
EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2.
Intracellular MUC1 peptides inhibit cancer progression.
Differential effects of EGFR ligands on endocytic sorting of the receptor.
ARAP1 regulates endocytosis of EGFR.
Cardiac ErbB-1/ErbB-2 mutant expression in young adult mice leads to cardiac dysfunction.
Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation.
Sensitivity of epidermal growth factor receptor and ErbB2 exon 20 insertion mutants to Hsp90 inhibition.
Acquired resistance to erlotinib in A-431 epidermoid cancer cells requires down-regulation of MMAC1/PTEN and up-regulation of phosphorylated Akt.
Progesterone receptors upregulate Wnt-1 to induce epidermal growth factor receptor transactivation and c-Src-dependent sustained activation of Erk1/2 mitogen-activated protein kinase in breast cancer cells.
Sensitivity of breast cancer cells to erlotinib depends on cyclin-dependent kinase 2 activity.
Diacylglycerol kinase delta regulates protein kinase C and epidermal growth factor receptor signaling.
Intersectin regulates epidermal growth factor receptor endocytosis, ubiquitylation, and signaling.
Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast.
The mucin Muc4 potentiates neuregulin signaling by increasing the cell-surface populations of ErbB2 and ErbB3.
Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1.
Sulindac metabolites inhibit epidermal growth factor receptor activation and expression.
Combining lapatinib (GW572016), a small molecule inhibitor of ErbB1 and ErbB2 tyrosine kinases, with therapeutic anti-ErbB2 antibodies enhances apoptosis of ErbB2-overexpressing breast cancer cells.
Functional effects of glycosylation at Asn-579 of the epidermal growth factor receptor.
Surface charge and hydrophobicity determine ErbB2 binding to the Hsp90 chaperone complex.
Ligand regulates epidermal growth factor receptor kinase specificity: activation increases preference for GAB1 and SHC versus autophosphorylation sites.
Alix/AIP1 antagonizes epidermal growth factor receptor downregulation by the Cbl-SETA/CIN85 complex.
Adhesion-mediated squamous cell carcinoma survival through ligand-independent activation of epidermal growth factor receptor.
Geldanamycin-associated inhibition of intracellular trafficking is attributed to a co-purified activity.
Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments.
Epidermal growth factor receptor signaling intensity determines intracellular protein interactions, ubiquitination, and internalization.
Profiling receptor tyrosine kinase activation by using Ab microarrays.
1,25-Dihydroxyvitamin D down-regulates cell membrane growth- and nuclear growth-promoting signals by the epidermal growth factor receptor.
Metalloproteinases stimulate ErbB-dependent ERK signaling in human skin organ culture.
Epidermal growth factor receptor signaling and the invasive phenotype of ovarian carcinoma cells.
Epidermal growth factor receptor signaling and the invasive phenotype of ovarian carcinoma cells.
Tyrosine-phosphorylated plakoglobin is associated with desmogleins but not desmoplakin after epidermal growth factor receptor activation.
Epidermal growth factor receptor vIII enhances tumorigenicity in human breast cancer.
Intracellular retention and degradation of the epidermal growth factor receptor, two distinct processes mediated by benzoquinone ansamycins.
Geldanamycin induces ErbB-2 degradation by proteolytic fragmentation.
A cytosolic domain of the erythropoietin receptor contributes to endoplasmic reticulum-associated degradation.
A cytosolic domain of the erythropoietin receptor contributes to endoplasmic reticulum-associated degradation.
A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera.
A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera.
Acevedo-Díaz A, Morales-Cabán BM, Zayas-Santiago A, Martínez-Montemayor MM, Suárez-Arroyo IJ
Cancers 2022 Jun 5;14(11)
Cancers 2022 Jun 5;14(11)
EGFR-targeted fluorescent imaging using the da Vinci® Firefly™ camera for gallbladder cancer.
Choi JH, Kang CM, Park JY
World journal of surgical oncology 2022 Jun 15;20(1):201
World journal of surgical oncology 2022 Jun 15;20(1):201
EGFR Transgene Stimulates Spontaneous Formation of MCF7 Breast Cancer Cells Spheroids with Partly Loss of HER3 Receptor.
Troitskaya O, Novak D, Nushtaeva A, Savinkova M, Varlamov M, Ermakov M, Richter V, Koval O
International journal of molecular sciences 2021 Nov 29;22(23)
International journal of molecular sciences 2021 Nov 29;22(23)
Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells.
Gerosa L, Chidley C, Fröhlich F, Sanchez G, Lim SK, Muhlich J, Chen JY, Vallabhaneni S, Baker GJ, Schapiro D, Atanasova MI, Chylek LA, Shi T, Yi L, Nicora CD, Claas A, Ng TSC, Kohler RH, Lauffenburger DA, Weissleder R, Miller MA, Qian WJ, Wiley HS, Sorger PK
Cell systems 2020 Nov 18;11(5):478-494.e9
Cell systems 2020 Nov 18;11(5):478-494.e9
Receptor-based mechanism of relative sensing and cell memory in mammalian signaling networks.
Lyashenko E, Niepel M, Dixit PD, Lim SK, Sorger PK, Vitkup D
eLife 2020 Jan 21;9
eLife 2020 Jan 21;9
Maximum Entropy Framework for Predictive Inference of Cell Population Heterogeneity and Responses in Signaling Networks.
Dixit PD, Lyashenko E, Niepel M, Vitkup D
Cell systems 2020 Feb 26;10(2):204-212.e8
Cell systems 2020 Feb 26;10(2):204-212.e8
Breast Cancer Cell Invasion into a Three Dimensional Tumor-Stroma Microenvironment.
Truong D, Puleo J, Llave A, Mouneimne G, Kamm RD, Nikkhah M
Scientific reports 2016 Sep 28;6:34094
Scientific reports 2016 Sep 28;6:34094
Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis.
Elkin SR, Oswald NW, Reed DK, Mettlen M, MacMillan JB, Schmid SL
Traffic (Copenhagen, Denmark) 2016 Oct;17(10):1139-49
Traffic (Copenhagen, Denmark) 2016 Oct;17(10):1139-49
EGFR Overexpressed in Colonic Neoplasia Can be Detected on Wide-Field Endoscopic Imaging.
Zhou J, Joshi BP, Duan X, Pant A, Qiu Z, Kuick R, Owens SR, Wang TD
Clinical and translational gastroenterology 2015 Jul 16;6(7):e101
Clinical and translational gastroenterology 2015 Jul 16;6(7):e101
Surfactant protein D suppresses lung cancer progression by downregulation of epidermal growth factor signaling.
Hasegawa Y, Takahashi M, Ariki S, Asakawa D, Tajiri M, Wada Y, Yamaguchi Y, Nishitani C, Takamiya R, Saito A, Uehara Y, Hashimoto J, Kurimura Y, Takahashi H, Kuroki Y
Oncogene 2015 Feb 12;34(7):838-45
Oncogene 2015 Feb 12;34(7):838-45
mMAPS: a flow-proteometric technique to analyze protein-protein interactions in individual signaling complexes.
Chou CK, Lee HH, Tsou PH, Chen CT, Hsu JM, Yamaguchi H, Wang YN, Lee HJ, Hsu JL, Lee JF, Kameoka J, Hung MC
Science signaling 2014 Mar 4;7(315):rs1
Science signaling 2014 Mar 4;7(315):rs1
Internalization mechanisms of the epidermal growth factor receptor after activation with different ligands.
Henriksen L, Grandal MV, Knudsen SL, van Deurs B, Grøvdal LM
PloS one 2013;8(3):e58148
PloS one 2013;8(3):e58148
Propranolol restricts the mobility of single EGF-receptors on the cell surface before their internalization.
Otero C, Linke M, Sanchez P, González A, Schaap IA
PloS one 2013;8(12):e83086
PloS one 2013;8(12):e83086
Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays.
Pötz O, Schneiderhan-Marra N, Henzler T, Herget T, Joos TO
Methods in molecular biology (Clifton, N.J.) 2012;795:191-202
Methods in molecular biology (Clifton, N.J.) 2012;795:191-202
Monitoring the size and lateral dynamics of ErbB1 enriched membrane domains through live cell plasmon coupling microscopy.
Rong G, Reinhard BM
PloS one 2012;7(3):e34175
PloS one 2012;7(3):e34175
A Miniaturized Ligand Binding Assay for EGFR.
Schwenk JM, Poetz O, Zeillinger R, Joos TO
International journal of proteomics 2012;2012:247059
International journal of proteomics 2012;2012:247059
Differential roles of Grb2 and AP-2 in p38 MAPK- and EGF-induced EGFR internalization.
Grandal MV, Grøvdal LM, Henriksen L, Andersen MH, Holst MR, Madshus IH, van Deurs B
Traffic (Copenhagen, Denmark) 2012 Apr;13(4):576-85
Traffic (Copenhagen, Denmark) 2012 Apr;13(4):576-85
Superior antitumoral activity of dimerized targeted single-chain TRAIL fusion proteins under retention of tumor selectivity.
Siegemund M, Pollak N, Seifert O, Wahl K, Hanak K, Vogel A, Nussler AK, Göttsch D, Münkel S, Bantel H, Kontermann RE, Pfizenmaier K
Cell death & disease 2012 Apr 12;3(4):e295
Cell death & disease 2012 Apr 12;3(4):e295
Novel membrane-associated androgen receptor splice variant potentiates proliferative and survival responses in prostate cancer cells.
Yang X, Guo Z, Sun F, Li W, Alfano A, Shimelis H, Chen M, Brodie AM, Chen H, Xiao Z, Veenstra TD, Qiu Y
The Journal of biological chemistry 2011 Oct 14;286(41):36152-60
The Journal of biological chemistry 2011 Oct 14;286(41):36152-60
Pnck induces ligand-independent EGFR degradation by probable perturbation of the Hsp90 chaperone complex.
Deb TB, Zuo AH, Wang Y, Barndt RJ, Cheema AK, Sengupta S, Coticchia CM, Johnson MD
American journal of physiology. Cell physiology 2011 May;300(5):C1139-54
American journal of physiology. Cell physiology 2011 May;300(5):C1139-54
High speed digital protein interaction analysis using microfluidic single molecule detection system.
Chou CK, Jing N, Yamaguchi H, Tsou PH, Lee HH, Chen CT, Wang YN, Hong S, Su C, Kameoka J, Hung MC
Lab on a chip 2010 Jul 21;10(14):1793-8
Lab on a chip 2010 Jul 21;10(14):1793-8
IL-24 is expressed during wound repair and inhibits TGFalpha-induced migration and proliferation of keratinocytes.
Poindexter NJ, Williams RR, Powis G, Jen E, Caudle AS, Chada S, Grimm EA
Experimental dermatology 2010 Aug;19(8):714-22
Experimental dermatology 2010 Aug;19(8):714-22
EGFR and EGFRvIII interact with PUMA to inhibit mitochondrial translocalization of PUMA and PUMA-mediated apoptosis independent of EGFR kinase activity.
Zhu H, Cao X, Ali-Osman F, Keir S, Lo HW
Cancer letters 2010 Aug 1;294(1):101-10
Cancer letters 2010 Aug 1;294(1):101-10
Spatial control of EGF receptor activation by reversible dimerization on living cells.
Chung I, Akita R, Vandlen R, Toomre D, Schlessinger J, Mellman I
Nature 2010 Apr 1;464(7289):783-7
Nature 2010 Apr 1;464(7289):783-7
EGFRvIII-induced estrogen-independence, tamoxifen-resistance phenotype correlates with PgR expression and modulation of apoptotic molecules in breast cancer.
Zhang Y, Su H, Rahimi M, Tochihara R, Tang C
International journal of cancer 2009 Nov 1;125(9):2021-8
International journal of cancer 2009 Nov 1;125(9):2021-8
Nuclear EGFR is required for cisplatin resistance and DNA repair.
Hsu SC, Miller SA, Wang Y, Hung MC
American journal of translational research 2009 Mar 8;1(3):249-58
American journal of translational research 2009 Mar 8;1(3):249-58
EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2.
Klessner JL, Desai BV, Amargo EV, Getsios S, Green KJ
Molecular biology of the cell 2009 Jan;20(1):328-37
Molecular biology of the cell 2009 Jan;20(1):328-37
Intracellular MUC1 peptides inhibit cancer progression.
Bitler BG, Menzl I, Huerta CL, Sands B, Knowlton W, Chang A, Schroeder JA
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 1;15(1):100-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 1;15(1):100-9
Differential effects of EGFR ligands on endocytic sorting of the receptor.
Roepstorff K, Grandal MV, Henriksen L, Knudsen SL, Lerdrup M, Grøvdal L, Willumsen BM, van Deurs B
Traffic (Copenhagen, Denmark) 2009 Aug;10(8):1115-27
Traffic (Copenhagen, Denmark) 2009 Aug;10(8):1115-27
ARAP1 regulates endocytosis of EGFR.
Yoon HY, Lee JS, Randazzo PA
Traffic (Copenhagen, Denmark) 2008 Dec;9(12):2236-52
Traffic (Copenhagen, Denmark) 2008 Dec;9(12):2236-52
Cardiac ErbB-1/ErbB-2 mutant expression in young adult mice leads to cardiac dysfunction.
Rajagopalan V, Zucker IH, Jones JA, Carlson M, Ma YJ
American journal of physiology. Heart and circulatory physiology 2008 Aug;295(2):H543-54
American journal of physiology. Heart and circulatory physiology 2008 Aug;295(2):H543-54
Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation.
Umebayashi K, Stenmark H, Yoshimori T
Molecular biology of the cell 2008 Aug;19(8):3454-62
Molecular biology of the cell 2008 Aug;19(8):3454-62
Sensitivity of epidermal growth factor receptor and ErbB2 exon 20 insertion mutants to Hsp90 inhibition.
Xu W, Soga S, Beebe K, Lee MJ, Kim YS, Trepel J, Neckers L
British journal of cancer 2007 Sep 17;97(6):741-4
British journal of cancer 2007 Sep 17;97(6):741-4
Acquired resistance to erlotinib in A-431 epidermoid cancer cells requires down-regulation of MMAC1/PTEN and up-regulation of phosphorylated Akt.
Yamasaki F, Johansen MJ, Zhang D, Krishnamurthy S, Felix E, Bartholomeusz C, Aguilar RJ, Kurisu K, Mills GB, Hortobagyi GN, Ueno NT
Cancer research 2007 Jun 15;67(12):5779-88
Cancer research 2007 Jun 15;67(12):5779-88
Progesterone receptors upregulate Wnt-1 to induce epidermal growth factor receptor transactivation and c-Src-dependent sustained activation of Erk1/2 mitogen-activated protein kinase in breast cancer cells.
Faivre EJ, Lange CA
Molecular and cellular biology 2007 Jan;27(2):466-80
Molecular and cellular biology 2007 Jan;27(2):466-80
Sensitivity of breast cancer cells to erlotinib depends on cyclin-dependent kinase 2 activity.
Yamasaki F, Zhang D, Bartholomeusz C, Sudo T, Hortobagyi GN, Kurisu K, Ueno NT
Molecular cancer therapeutics 2007 Aug;6(8):2168-77
Molecular cancer therapeutics 2007 Aug;6(8):2168-77
Diacylglycerol kinase delta regulates protein kinase C and epidermal growth factor receptor signaling.
Crotty T, Cai J, Sakane F, Taketomi A, Prescott SM, Topham MK
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 17;103(42):15485-90
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 17;103(42):15485-90
Intersectin regulates epidermal growth factor receptor endocytosis, ubiquitylation, and signaling.
Martin NP, Mohney RP, Dunn S, Das M, Scappini E, O'Bryan JP
Molecular pharmacology 2006 Nov;70(5):1643-53
Molecular pharmacology 2006 Nov;70(5):1643-53
Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast.
Kim YS, Bhandari R, Cochran JR, Kuriyan J, Wittrup KD
Proteins 2006 Mar 1;62(4):1026-35
Proteins 2006 Mar 1;62(4):1026-35
The mucin Muc4 potentiates neuregulin signaling by increasing the cell-surface populations of ErbB2 and ErbB3.
Funes M, Miller JK, Lai C, Carraway KL 3rd, Sweeney C
The Journal of biological chemistry 2006 Jul 14;281(28):19310-9
The Journal of biological chemistry 2006 Jul 14;281(28):19310-9
Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1.
Lo HW, Ali-Seyed M, Wu Y, Bartholomeusz G, Hsu SC, Hung MC
Journal of cellular biochemistry 2006 Aug 15;98(6):1570-83
Journal of cellular biochemistry 2006 Aug 15;98(6):1570-83
Sulindac metabolites inhibit epidermal growth factor receptor activation and expression.
Pangburn HA, Kraus H, Ahnen DJ, Rice PL
Journal of carcinogenesis 2005 Sep 2;4:16
Journal of carcinogenesis 2005 Sep 2;4:16
Combining lapatinib (GW572016), a small molecule inhibitor of ErbB1 and ErbB2 tyrosine kinases, with therapeutic anti-ErbB2 antibodies enhances apoptosis of ErbB2-overexpressing breast cancer cells.
Xia W, Gerard CM, Liu L, Baudson NM, Ory TL, Spector NL
Oncogene 2005 Sep 15;24(41):6213-21
Oncogene 2005 Sep 15;24(41):6213-21
Functional effects of glycosylation at Asn-579 of the epidermal growth factor receptor.
Whitson KB, Whitson SR, Red-Brewer ML, McCoy AJ, Vitali AA, Walker F, Johns TG, Beth AH, Staros JV
Biochemistry 2005 Nov 15;44(45):14920-31
Biochemistry 2005 Nov 15;44(45):14920-31
Surface charge and hydrophobicity determine ErbB2 binding to the Hsp90 chaperone complex.
Xu W, Yuan X, Xiang Z, Mimnaugh E, Marcu M, Neckers L
Nature structural & molecular biology 2005 Feb;12(2):120-6
Nature structural & molecular biology 2005 Feb;12(2):120-6
Ligand regulates epidermal growth factor receptor kinase specificity: activation increases preference for GAB1 and SHC versus autophosphorylation sites.
Fan YX, Wong L, Deb TB, Johnson GR
The Journal of biological chemistry 2004 Sep 10;279(37):38143-50
The Journal of biological chemistry 2004 Sep 10;279(37):38143-50
Alix/AIP1 antagonizes epidermal growth factor receptor downregulation by the Cbl-SETA/CIN85 complex.
Schmidt MHH, Hoeller D, Yu J, Furnari FB, Cavenee WK, Dikic I, Bögler O
Molecular and cellular biology 2004 Oct;24(20):8981-93
Molecular and cellular biology 2004 Oct;24(20):8981-93
Adhesion-mediated squamous cell carcinoma survival through ligand-independent activation of epidermal growth factor receptor.
Shen X, Kramer RH
The American journal of pathology 2004 Oct;165(4):1315-29
The American journal of pathology 2004 Oct;165(4):1315-29
Geldanamycin-associated inhibition of intracellular trafficking is attributed to a co-purified activity.
Barzilay E, Ben-Califa N, Supino-Rosin L, Kashman Y, Hirschberg K, Elazar Z, Neumann D
The Journal of biological chemistry 2004 Feb 20;279(8):6847-52
The Journal of biological chemistry 2004 Feb 20;279(8):6847-52
Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments.
Cochran JR, Kim YS, Olsen MJ, Bhandari R, Wittrup KD
Journal of immunological methods 2004 Apr;287(1-2):147-58
Journal of immunological methods 2004 Apr;287(1-2):147-58
Epidermal growth factor receptor signaling intensity determines intracellular protein interactions, ubiquitination, and internalization.
Schmidt MHH, Furnari FB, Cavenee WK, Bögler O
Proceedings of the National Academy of Sciences of the United States of America 2003 May 27;100(11):6505-10
Proceedings of the National Academy of Sciences of the United States of America 2003 May 27;100(11):6505-10
Profiling receptor tyrosine kinase activation by using Ab microarrays.
Nielsen UB, Cardone MH, Sinskey AJ, MacBeath G, Sorger PK
Proceedings of the National Academy of Sciences of the United States of America 2003 Aug 5;100(16):9330-5
Proceedings of the National Academy of Sciences of the United States of America 2003 Aug 5;100(16):9330-5
1,25-Dihydroxyvitamin D down-regulates cell membrane growth- and nuclear growth-promoting signals by the epidermal growth factor receptor.
Cordero JB, Cozzolino M, Lu Y, Vidal M, Slatopolsky E, Stahl PD, Barbieri MA, Dusso A
The Journal of biological chemistry 2002 Oct 11;277(41):38965-71
The Journal of biological chemistry 2002 Oct 11;277(41):38965-71
Metalloproteinases stimulate ErbB-dependent ERK signaling in human skin organ culture.
Stoll SW, Kansra S, Elder JT
The Journal of biological chemistry 2002 Jul 26;277(30):26839-45
The Journal of biological chemistry 2002 Jul 26;277(30):26839-45
Epidermal growth factor receptor signaling and the invasive phenotype of ovarian carcinoma cells.
Alper O, Bergmann-Leitner ES, Bennett TA, Hacker NF, Stromberg K, Stetler-Stevenson WG
Journal of the National Cancer Institute 2001 Sep 19;93(18):1375-84
Journal of the National Cancer Institute 2001 Sep 19;93(18):1375-84
Epidermal growth factor receptor signaling and the invasive phenotype of ovarian carcinoma cells.
Alper O, Bergmann-Leitner ES, Bennett TA, Hacker NF, Stromberg K, Stetler-Stevenson WG
Journal of the National Cancer Institute 2001 Sep 19;93(18):1375-84
Journal of the National Cancer Institute 2001 Sep 19;93(18):1375-84
Tyrosine-phosphorylated plakoglobin is associated with desmogleins but not desmoplakin after epidermal growth factor receptor activation.
Gaudry CA, Palka HL, Dusek RL, Huen AC, Khandekar MJ, Hudson LG, Green KJ
The Journal of biological chemistry 2001 Jul 6;276(27):24871-80
The Journal of biological chemistry 2001 Jul 6;276(27):24871-80
Epidermal growth factor receptor vIII enhances tumorigenicity in human breast cancer.
Tang CK, Gong XQ, Moscatello DK, Wong AJ, Lippman ME
Cancer research 2000 Jun 1;60(11):3081-7
Cancer research 2000 Jun 1;60(11):3081-7
Intracellular retention and degradation of the epidermal growth factor receptor, two distinct processes mediated by benzoquinone ansamycins.
Supino-Rosin L, Yoshimura A, Yarden Y, Elazar Z, Neumann D
The Journal of biological chemistry 2000 Jul 21;275(29):21850-5
The Journal of biological chemistry 2000 Jul 21;275(29):21850-5
Geldanamycin induces ErbB-2 degradation by proteolytic fragmentation.
Tikhomirov O, Carpenter G
The Journal of biological chemistry 2000 Aug 25;275(34):26625-31
The Journal of biological chemistry 2000 Aug 25;275(34):26625-31
A cytosolic domain of the erythropoietin receptor contributes to endoplasmic reticulum-associated degradation.
Supino-Rosin L, Yoshimura A, Altaratz H, Neumann D
European journal of biochemistry 1999 Jul;263(2):410-9
European journal of biochemistry 1999 Jul;263(2):410-9
A cytosolic domain of the erythropoietin receptor contributes to endoplasmic reticulum-associated degradation.
Supino-Rosin L, Yoshimura A, Altaratz H, Neumann D
European journal of biochemistry 1999 Jul;263(2):410-9
European journal of biochemistry 1999 Jul;263(2):410-9
A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera.
Baron AT, Lafky JM, Connolly DC, Peoples J, O'Kane DJ, Suman VJ, Boardman CH, Podratz KC, Maihle NJ
Journal of immunological methods 1998 Oct 1;219(1-2):23-43
Journal of immunological methods 1998 Oct 1;219(1-2):23-43
A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera.
Baron AT, Lafky JM, Connolly DC, Peoples J, O'Kane DJ, Suman VJ, Boardman CH, Podratz KC, Maihle NJ
Journal of immunological methods 1998 Oct 1;219(1-2):23-43
Journal of immunological methods 1998 Oct 1;219(1-2):23-43
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Epidermal Growth Factor Receptor using Epidermal Growth Factor Receptor Monoclonal Antibody (Product # MA5-13319) on A431 Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EGFR (green) showing staining in the cytoplasm and membrane of A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Epidermal Growth Factor Receptor monoclonal antibody (Product # MA5-13319) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EGFR (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Epidermal Growth Factor Receptor monoclonal antibody (Product # MA5-13319) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Submitted by
- Invitrogen Antibodies (provider)
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- Immunofluorescence analysis of EGFR was performed using 90% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EGFR Mouse monoclonal antibody (Product # MA5-13319) at 5 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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- Immunofluorescence analysis of EGFR was done on 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled EGFR (199.12) Mouse Monoclonal Antibody (Product # MA5-13319) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
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- Immunofluorescence analysis of EGFR was performed using 70% confluent log phase A-431 cells (WIld type, panels a,d), CAS9 control (panels b,e) and EGFR Knockout (panels c,f). The cells were fixed, permeabilized, and labelled with EGFR Mouse Monoclonal Antibody(Product # MA5-13319, 5 µg/mL), followed by Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175, 1:2000). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938) and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Loss of signal was observed in EGFR Knockout cells (panel c,f) confirming specificity of the antibody to EGFR(green). The images were captured at 60X magnification.
Supportive validation
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- Flow cytometry analysis of EGFR was done on A-431 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with EGFR Mouse Monoclonal Antibody (MA513319, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
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- Immunoprecipitation of Epidermal Growth Factor Receptor using Epidermal Growth Factor Receptor Monoclonal Antibody (Product # MA5-13319) on Native Human A431 Cells.
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- Figure 8 Investigation of EGFR and pEGFR. ( A ) Cells were stained for EGFR (red), pEGFR (green), and nuclei (blue) (scale bar: 20 mum). ( B ) Representative images of EGFR clusters with corresponding heat maps of relative intensities. ( C ) (+) EGF demonstrated significantly lower EGFR to cell area ratio. ( D , E ) (+) EGF displayed significantly higher pEGFR to cell area ratio and pEGFR to EGFR area ratio ( p < 0.05 calculated from Student's t -test with n > 18 from more than three devices for each condition).
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- Figure 1 Characterization of MCF7-derived MCF7-EGFR cells. ( A ) Flow cytometry analysis of surface EGFR expression. Cells from gate P1 were sorted and cultivated as MCF7-EGFR cells. ( B ) Western blot of total cellular EGFR (typical image). Tubulin was used as a loading control.
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- Figure 4 SCAMP3 colocalizes with EGFR after receptor internalization. Cells were stimulated with 10 ng/mL EGF at the indicated time points to evaluate the location of SCAMP3 and EGFR using confocal microscopy. Representative images of the internalization assay and fluorescence quantification of SCAMP3, EGFR, and their colocalization in ( A - C ) SUM-149 and ( D - F ) MDA-MB-468 cells. The nuclei were stained with DAPI (blue). Secondary antibodies conjugated to Alexa 488 (green) and Alexa 594 (red) were used to detect SCAMP3 and EGFR, respectively. The micrographs were taken at a magnification of 60x using confocal microscopy. The white arrows indicate the colocalization of EGFR and SCAMP3 in the zoom images. The zoom-in of each image is shown in white squares and each has equal dimensions. Scale bar = 20 um. Total cell fluorescence and colocalization area analyses were performed in 20 cells from three experiments using Image J. Colocalization was calculated as the ratio of the colocalization fluorescence area to the total cell fluorescence area. One way or two-way ANOVA; * p
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- Characterization of gallbladder tumor cell lines. A Western blot analysis of EGFR expression in NOZ cells (right lane) and HEK293 cells (left lane). B Flow cytometric analysis of fluorescent anti-EGFR bounding in NOZ cells (blue line) and HEK293 cells (red line). C Labeling of NOZ and HEK293 cells with fluorescent anti-EGFR (x40 water immersion objective with the LSM 700 confocal microscope, using 405- and 488-nm lasers). The white scale bar represents 10 mum
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- Figure 1--figure supplement 1. Representative immunofluorescence stains of Akt phosphorylation and cell surface EGF receptor levels. Representative immunofluorescence images of MCF10A cells used for the generation of quantitative data on Akt phosphorylation (pAkt) and cell surface EGFR (sEGFR). (left) MCF10A cells 5 min after control treatment ( a ), or treatment with 1 ng/ml ( b ) or 100 ng/ml of EGF stained for pAKT. (right) MCF 10A cells 180 min after control treatment (a), or treatment with 1 ng/ml (b) or 100 ng/ml of EGF stained for sEGFR. Pseudocolors: blue: nuclei; green: cytoplasm; red: pAKT (left panels) or sEGFR (right panels).