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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- MAB3095 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Fibulin 5/DANCE Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Fibulin 5/DANCE in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references LTBP-2 competes with tropoelastin for binding to fibulin-5 and heparin, and is a negative modulator of elastinogenesis.
Fibulin-5 inhibits hepatocellular carcinoma cell migration and invasion by down-regulating matrix metalloproteinase-7 expression.
Fibulin-5 suppresses lung cancer invasion by inhibiting matrix metalloproteinase-7 expression.
Fibulin-5 binds human smooth-muscle cells through alpha5beta1 and alpha4beta1 integrins, but does not support receptor activation.
Sideek MA, Menz C, Parsi MK, Gibson MA
Matrix biology : journal of the International Society for Matrix Biology 2014 Feb;34:114-23
Matrix biology : journal of the International Society for Matrix Biology 2014 Feb;34:114-23
Fibulin-5 inhibits hepatocellular carcinoma cell migration and invasion by down-regulating matrix metalloproteinase-7 expression.
Tu K, Dou C, Zheng X, Li C, Yang W, Yao Y, Liu Q
BMC cancer 2014 Dec 12;14:938
BMC cancer 2014 Dec 12;14:938
Fibulin-5 suppresses lung cancer invasion by inhibiting matrix metalloproteinase-7 expression.
Yue W, Sun Q, Landreneau R, Wu C, Siegfried JM, Yu J, Zhang L
Cancer research 2009 Aug 1;69(15):6339-46
Cancer research 2009 Aug 1;69(15):6339-46
Fibulin-5 binds human smooth-muscle cells through alpha5beta1 and alpha4beta1 integrins, but does not support receptor activation.
Lomas AC, Mellody KT, Freeman LJ, Bax DV, Shuttleworth CA, Kielty CM
The Biochemical journal 2007 Aug 1;405(3):417-28
The Biochemical journal 2007 Aug 1;405(3):417-28
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Fibulin 5/DANCE by Western Blot. Western blot shows lysates of human heart tissue, human lung tissue, and human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibulin 5/DANCE Monoclonal Antibody (Catalog # MAB3095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Fibulin 5 (Catalog # 3095-FB) (5 ng/lane) was included. A specific band was detected for Fibulin 5/DANCE at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
