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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- ABIN238330 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN238330, RRID:AB_10955189
- Product name
- anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 20 (CEACAM20) antibody
- Antibody type
- Monoclonal
- Antigen
- genetic immunisation with cDNA encoding human CEACAM20
- Description
- Protein G
- Reactivity
- Human
- Isotype
- IgG
- Antibody clone number
- HT-12D8
- Vial size
- 100 μg
- Storage
- short term: 2°C - 8°C, long term: -20°C
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified HT-12D8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified HT-12D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Spectral Confocal Microscopy of CHO cells using HT-12D8. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: ?-myc; CEACAM20: ?-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified HT-12D8 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM20 transfected cells (red curve).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using HT-12D8. BOSC23 cells were transiently trans-fected with an expression vector encoding either CEACAM20 (red curve) or an irrelevant protein (control transfectant). Binding of HT-12D8 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM20 transfected cells
