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- Western blot [1]
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- Flow cytometry [2]
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- Product number
- DM1231 - Provider product page
- Provider
- OriGene
- Product name
- CEACAM20 mouse monoclonal antibody, clone HT-12D8, Purified
- Antibody type
- Monoclonal
- Description
- CEACAM20 mouse monoclonal antibody, clone HT-12D8, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CEACAM20
- Isotype
- IgG
- Antibody clone number
- HT-12D8
- Vial size
- 100 µg
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure.4: SDS-PAGE analysis of purified HT-12D8 monoclonal antibody. Lane 1: Molecular weight marker, Lane 2: 2 ug of purified HT-12D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure.2: Spectral Confocal Microscopy of CHO cells using HT-12D8 antibody . CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure.3: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8:Tet2; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified HT-12D8 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM20 transfected cells (red curve).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure.1: FACS analysis of BOSC23 cells using HT-12D8 antibody . BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM20 (Red curve) or an irrelevant protein (control transfectant). Binding of HT-12D8 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM20 transfected cells.
- Validation comment
- FC
