PAB28419

antibody from Abnova Corporation

Targeting: STX5 SED5, STX5A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28419

Provider

Abnova Corporation

Product name

STX5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant STX5.

Antigen sequence

QIAQLQDFVRAKGSQSGRHLQTHSNTIVVSLQSKL
ASMSNDFKSVLEVRTENLKQQRSRREQFSRAPVSA
LPLAPNHLGGGAVVLGAESHASKDVAIDMMDSRTS
QQLQLIDEQDSYIQSRADTMQNIESTIVELGSIFQ
QLAHMV


Isotype

IgG

Storage

Store at 4 °C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of HEK293T cell lysate using STX5 polyclonal antibody (Cat # PAB28419).Lane 1: Negative control (vector only)Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of U-2 OS cell line with STX5 polyclonal antibody (Cat # PAB28419) shows positivity in nucleus but not nucleoli and golgi apparatus. Fixation/Permeabilization: PFA/Triton X-100

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human pancreas with STX5 polyclonal antibody (Cat # PAB28419) shows strong cytoplasmic positivity in exocrine glandular cells.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)