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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-16060 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal CYFIP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CYFIP1 Antibody [NBP2-16060] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CYFIP1 Antibody [NBP2-16060] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CYFIP1 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CYFIP1 Antibody [NBP2-16060] - Immunoprecipitation of CYFIP1 protein from HeLa whole cell extracts using 5 ug of CYFIP1 antibody [N1N2], N-term. Western blot analysis was performed using CYFIP1 antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CYFIP1 Antibody [NBP2-16060] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
