PA5-31984

antibody from Invitrogen Antibodies

Targeting: CYFIP1 KIAA0068, P140SRA-1, SHYC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31984

Provider

Invitrogen Antibodies

Product name

CYFIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, mouse brain, rat brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CYFIP1 using 30 µg of BCL-1 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a CYFIP1 polyclonal antibody (Product # PA5-31984) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CYFIP1 was performed by separating 50 µg of various tissue extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CYFIP1 Polyclonal Antibody (Product # PA5-31984) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CYFIP1 Polyclonal Antibody (Product # PA5-31984). Sample (30 µg of whole cell lysate). Lane A: HeLa. 5% SDS PAGE. CYFIP1 Polyclonal Antibody (Product # PA5-31984) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CYFIP1 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CYFIP1 Polyclonal Antibody (Product # PA5-31984) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human hepatoma, using CYFIP1 (Product # PA5-31984) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CYFIP1 was performed in HeLa whole cell extracts using 5 µg of CYFIP1 Polyclonal Antibody (Product # PA5-31984). Samples were transferred to a membrane and probed with CYFIP1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.