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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB3209-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Primate BMP-9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and primate BMP-9 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human BMP-1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8, or -10 is observed. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse (rm) BMP-9 and 10% cross-reactivity with rmBMP-10 is observed.
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9UK05- Isotype
- IgG
- Antibody clone number
- 360107
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Identification of rare sequence variation underlying heritable pulmonary arterial hypertension.
BMP-9 interferes with liver regeneration and promotes liver fibrosis.
A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10.
Rapid Activation of Bone Morphogenic Protein 9 by Receptor-mediated Displacement of Pro-domains.
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.
Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Gräf S, Haimel M, Bleda M, Hadinnapola C, Southgate L, Li W, Hodgson J, Liu B, Salmon RM, Southwood M, Machado RD, Martin JM, Treacy CM, Yates K, Daugherty LC, Shamardina O, Whitehorn D, Holden S, Aldred M, Bogaard HJ, Church C, Coghlan G, Condliffe R, Corris PA, Danesino C, Eyries M, Gall H, Ghio S, Ghofrani HA, Gibbs JSR, Girerd B, Houweling AC, Howard L, Humbert M, Kiely DG, Kovacs G, MacKenzie Ross RV, Moledina S, Montani D, Newnham M, Olschewski A, Olschewski H, Peacock AJ, Pepke-Zaba J, Prokopenko I, Rhodes CJ, Scelsi L, Seeger W, Soubrier F, Stein DF, Suntharalingam J, Swietlik EM, Toshner MR, van Heel DA, Vonk Noordegraaf A, Waisfisz Q, Wharton J, Wort SJ, Ouwehand WH, Soranzo N, Lawrie A, Upton PD, Wilkins MR, Trembath RC, Morrell NW
Nature communications 2018 Apr 12;9(1):1416
Nature communications 2018 Apr 12;9(1):1416
BMP-9 interferes with liver regeneration and promotes liver fibrosis.
Breitkopf-Heinlein K, Meyer C, König C, Gaitantzi H, Addante A, Thomas M, Wiercinska E, Cai C, Li Q, Wan F, Hellerbrand C, Valous NA, Hahnel M, Ehlting C, Bode JG, Müller-Bohl S, Klingmüller U, Altenöder J, Ilkavets I, Goumans MJ, Hawinkels LJ, Lee SJ, Wieland M, Mogler C, Ebert MP, Herrera B, Augustin H, Sánchez A, Dooley S, Ten Dijke P
Gut 2017 May;66(5):939-954
Gut 2017 May;66(5):939-954
A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10.
Ruiz S, Zhao H, Chandakkar P, Chatterjee PK, Papoin J, Blanc L, Metz CN, Campagne F, Marambaud P
Scientific reports 2016 Nov 22;5:37366
Scientific reports 2016 Nov 22;5:37366
Rapid Activation of Bone Morphogenic Protein 9 by Receptor-mediated Displacement of Pro-domains.
Kienast Y, Jucknischke U, Scheiblich S, Thier M, de Wouters M, Haas A, Lehmann C, Brand V, Bernicke D, Honold K, Lorenz S
The Journal of biological chemistry 2016 Feb 12;291(7):3395-410
The Journal of biological chemistry 2016 Feb 12;291(7):3395-410
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
Chen J, Liu H, Liu J, Qi J, Wei B, Yang J, Liang H, Chen Y, Chen J, Wu Y, Guo L, Zhu J, Zhao X, Peng T, Zhang Y, Chen S, Li X, Li D, Wang T, Pei D
Nature genetics 2013 Jan;45(1):34-42
Nature genetics 2013 Jan;45(1):34-42
Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.
van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P
The Journal of biological chemistry 2012 May 25;287(22):18551-61
The Journal of biological chemistry 2012 May 25;287(22):18551-61
Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Boström KI
Blood 2012 May 24;119(21):5037-47
Blood 2012 May 24;119(21):5037-47
BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
Ricard N, Ciais D, Levet S, Subileau M, Mallet C, Zimmers TA, Lee SJ, Bidart M, Feige JJ, Bailly S
Blood 2012 Jun 21;119(25):6162-71
Blood 2012 Jun 21;119(25):6162-71
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Herrera B, Inman GJ
BMC cell biology 2009 Mar 19;10:20
BMC cell biology 2009 Mar 19;10:20
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Alkaline Phosphatase Production Induced by BMP-9 and Neutralization by Human BMP-9 Antibody. Recombinant Human BMP-9 (Catalog # 3209-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse/Primate BMP-9 Monoclonal Antibody (Catalog # MAB3209). The ND50 is typically 0.01-0.04 µg/mL.
