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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [4]
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- Product number
- PA5-12459 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNRPD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse and non-human primate based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Impaired spliceosomal UsnRNP assembly leads to Sm mRNA down-regulation and Sm protein degradation.
Prusty AB, Meduri R, Prusty BK, Vanselow J, Schlosser A, Fischer U
The Journal of cell biology 2017 Aug 7;216(8):2391-2407
The Journal of cell biology 2017 Aug 7;216(8):2391-2407
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a SNRPD1 polyclonal antibody (Product # PA5-12459) in HL-60 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNRPD1 (arrow) using a SNRPD1 polyclonal antibody (Product # PA5-12459) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNRPD1 gene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue using a SNRPD1 polyclonal antibody (Product # PA5-12459), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HL-60 cells using a SNRPD1 polyclonal antibody (Product # PA5-12459) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Destabilization of Sm transcripts after extended inhibition of the late assembly phase. (A) Normalized fold change of transcripts encoding the indicated Sm proteins, SMN, and Gemin5, as well as U1 and U2 snRNAs in control cells (black bar) and SMN knockdown cells (gray bars), respectively, 144 h after doxycycline induction. (Top) Western blot analysis showing knockdown of SMN, with tubulin as loading control. (B) Normalized fold change of random transcripts (PI15, TOP3b, CPOX, hTR, and ASNS) that are unrelated to UsnRNP biogenesis as well as LSm encoding transcripts (LSm 2, 4,10, 11), PHAX and SNUP1. (C) qRT-PCR analysis of recovery of Sm encoding transcripts in control (black bars) and SMN knockdown (gray bars) cells upon treatment with 5-FU, an exosome inhibitor. Expression normalized to control cells treated with 5-FU. (Top) Western blot analysis showing knockdown of SMN, with tubulin as loading control. (D) Normalized fold change of transcripts encoding the indicated Sm proteins, SMN, and Gemin5, as well as U1 and U2 snRNAs in control cells (black bar) and SMN knockdown cells (gray bars) 168 h after doxycycline induction. (Top) Western blot analysis showing knockdown of SMN, with tubulin as loading control. Error bars indicate SE ( n = 3, three independent biological experiments). **, P < 0.0005; *, P < 0.05, Student's t test. GAPDH mRNA was used as a control for normalization.
