AP13062a

antibody from Abgent

Targeting: H2AC4 H2A/m, H2AFM, HIST1H2AB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP13062a

Provider

Abgent

Proper citation

Abgent Cat#AP13062a, RRID:AB_10821695

Product name

HIST1H2AB Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"HIST1H2AB Antibody (N-term) (Cat. #AP13062a) western blot analysis in K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HIST1H2AB antibody detected the HIST1H2AB protein (arrow)."

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

HIST1H2AB Antibody (N-term) (Cat. #AP13062a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H2AB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

HIST1H2AB Antibody (N-term) (Cat. #AP13062a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50