AP13062a

antibody from Abcepta

Targeting: H2AC4 H2A/m, H2AFM, HIST1H2AB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP13062a

Provider

Abcepta

Proper citation

Product name

HIST1H2AB Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"HIST1H2AB Antibody (N-term) (Cat. #AP13062a) western blot analysis in K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HIST1H2AB antibody detected the HIST1H2AB protein (arrow)."

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

HIST1H2AB Antibody (N-term) (Cat. #AP13062a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H2AB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

HIST1H2AB Antibody (N-term) (Cat. #AP13062a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50