M16777

antibody from Boster Biological Technology

Targeting: H2AC4 H2A/m, H2AFM, HIST1H2AB

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M16777

Provider

Boster Biological Technology

Product name

Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for HIST1H2ABHIST1H2AE detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat HIST1H2ABHIST1H2AE information: Molecular Weight: 14135 MW; Subcellular Localization: Nucleus. Chromosome.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

EOO-8

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human kidney, using Histone H2AZ Antibody(M16777)HIST1H2AB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H2AB Antibody (M16777)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image