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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- MA5-28990 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACBD6 Monoclonal Antibody (4A9D5E9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human ACBD6.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4A9D5E9
- Vial size
- 100 µL
- Concentration
- 5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACBD6 in Lane A: 293T Whole Cell Lysate (30 µg). Samples were probed using a ACBD6 Monoclonal Antibody (Product # MA5-28990) at a 1:500 dilution, followed by a Goat Anti-Mouse IgG (H+L), Dylight 800 Secondary Antibody at a 1:15000 dilution. Western blot was performed under reducing conditions. Predicted band size:31 kDa. Observed band size:35 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ACBD6 Monoclonal Antibody (4A9D5E9) (Product # MA5-28990) at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ACBD6 Immunoprecipitation using: Lane A: 0.5 mg hela Whole Cell Lysate 0.5 µL with ACBD6 Monoclonal Antibody (4A9D5E9) (Product # MA5-28990) and 15 µL of 50 % Protein G agarose. Primary antibody: ACBD6 Monoclonal Antibody (4A9D5E9), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7,500 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 31 kDa.
