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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00388677-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00388677-M01, RRID:AB_426149
- Product name
- NOTCH2NL monoclonal antibody (M01), clone 2G12-2A5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant NOTCH2NL.
- Antigen sequence
MCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQ
NGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPC
FVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQW
TDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKC
ETDVNECDIPGHCQHGGICLNLPGSYQCQCLQGFT
GQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCL
PETVRRGTELWERDREVWNGKEHDEN- Isotype
- IgG
- Antibody clone number
- 2G12-2A5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NOTCH2NL monoclonal antibody (M01), clone 2G12-2A5 Western Blot analysis of NOTCH2NL expression in HL-60 ( Cat # L014V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NOTCH2NL is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NOTCH2NL on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
