GTX24803

antibody from GeneTex

Targeting: PTK2 FADK, FAK, FAK1, PPP1R71

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX24803

Provider

GeneTex

Proper citation

GeneTex Cat#GTX24803, RRID:AB_380431

Product name

FAK (phospho Tyr397) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Drosophila, Feline, Xenopus, Zebrafish

Host

Rabbit

References

Submitted references

Glycine and succinic acid are effective indicators of the suppression of epithelial-mesenchymal transition by fucoxanthinol in colorectal cancer stem-like cells.

Terasaki M, Mima M, Kudoh S, Endo T, Maeda H, Hamada J, Osada K, Miyashita K, Mutoh M
Oncology reports 2018 Jul;40(1):414-424

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Suspension survival mediated by PP2A-STAT3-Col XVII determines tumour initiation and metastasis in cancer stem cells.

Liu CC, Lin SP, Hsu HS, Yang SH, Lin CH, Yang MH, Hung MC, Hung SC
Nature communications 2016 Jun 16;7:11798

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Mesenchymal Stem Cells Induce Directional Migration of Invasive Breast Cancer Cells through TGF-β.

McAndrews KM, McGrail DJ, Ravikumar N, Dawson MR
Scientific reports 2015 Nov 20;5:16941

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Alterations in ovarian cancer cell adhesion drive taxol resistance by increasing microtubule dynamics in a FAK-dependent manner.

McGrail DJ, Khambhati NN, Qi MX, Patel KS, Ravikumar N, Brandenburg CP, Dawson MR
Scientific reports 2015 Apr 17;5:9529

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Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells.

Hsu HY, Lin JH, Li CJ, Tsang SF, Tsai CH, Chyuan JH, Chiu SJ, Chuang SE
Evidence-based complementary and alternative medicine : eCAM 2012;2012:819632

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Peptide Competition: Extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were probed with 0.35 ?g/mL GTX24803 antibody following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho-tyrosine containing peptide (3), a phosphopeptide derived from the corresponding region of Pyk2 (4), or, the phosphopeptide immunogen (5).? The data show that only the peptide corresponding to GTX24803 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FAK [pY397] PSSA (GTX24803) analysis of CEF cells illustrate that only the phosphopeptide corresponding to FAK [pY397] blocks the Western blot signal.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Visualizing focal adhesion kinase with FAK [pY397] PSSA. HeLa cells were fixed and permeabilized using ice-cold 95% methanol. FAK [pY397] PSSA was labeled with Alexa Fluor? 488 dye (green fluorescence), and nuclei were counterstained with DAPI (blue fluorescence). The image was captured using a fluorescence microscope fitted with a 100¡Ñ oil immersion lens.