694002

antibody from BioLegend

Targeting: PTK2 FADK, FAK, FAK1, PPP1R71

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

694002

Provider

BioLegend

Proper citation

BioLegend Cat#694002, RRID:AB_2650722

Product name

Purified anti-FAK

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16060A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from RPMI8826, HeLa, PC3 and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-FAK (clone W16060A) antibody. Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.

Conjugate

Unconjugated