MA5-33154

antibody from Invitrogen Antibodies

Targeting: H2BC3 H2B/f, H2BFF, HIST1H2BB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-33154

Provider

Invitrogen Antibodies

Product name

H2BK20ac (type 1-B) Recombinant Rabbit Monoclonal Antibody (21F11)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

21F11

Vial size

100 µL

Concentration

0.9 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Acetyl-Histone H2B type 1-B (Lys20) using a Acetyl-Histone H2B type 1-B (Lys20) Monoclonal antibody (Product # MA5-33154) at a concentration of 0.135 µg/mL. Positive WB detected in: Hela whole cell lysate treated by 15mM sodium butyrate for 30min. All lanes: Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 15 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Acetyl-Histone H2B type 1-B (Lys20) in paraffin embedded Hela cells using a Acetyl-Histone H2B type 1-B (Lys20) monoclonal antibody (Product # MA5-33154) at a dilution of 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Acetyl-Histone H2B type 1-B (Lys20) in Hela cells (treated by 15mM sodium butyrate for 30min) using a Acetyl-Histone H2B type 1-B (Lys20) monoclonal antibody (Product # MA5-33154) at a dilution of 1:84. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cells were counter-stained with DAPI.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Acetyl-Histone H2B type 1-B (Lys20) in Hela cells using a monoclonal antibody (Product # MA5-33154) at a dilution of 1:50. The cells were fixed with 70% Ethyl alcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1:200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.