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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504530 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504530, RRID:AB_2622459
- Product name
- LHX1 (LIM1) mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)
- Antibody type
- Monoclonal
- Description
- LHX1 (LIM1) mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LHX1
- Isotype
- IgG
- Antibody clone number
- OTI2D3
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LHX1 (RC210977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LHX1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-LHX1 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-LHX1 mouse monoclonal antibody (TA504530) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LHX1(RC210977).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-LHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504530)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC210977 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LHX1 antibody(TA504530), and then analyzed by flow cytometry.
- Validation comment
- FC
