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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX106233 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX106233, RRID:AB_10722404
- Product name
- ERR alpha antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE GTX106233 diluted at 1:2000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 £gg whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 £gg of ERR alpha antibody (GTX106233) 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (GTX106233) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 £gg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.
