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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AP12085PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP12085PU-N, RRID:AB_1769781
- Product name
- anti MIB1 (N-term)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MIB.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- (LEFT) Western blot analysis using anti-Mib (N-term) Pab (Cat.#AP12085PU-N) at 1:300 to detect FLAG-tagged Mib in 293T cell lysate. Data courtesy of Dr. Olga Ossipova, Mt. Sinai School of Medicine. (RIGHT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Hela cells incubated with MIB Antibody (N-term) (Cat#AP12085PU-N) at a dilution of 1:20. Data courtesy of Dr. Vyacheslav Akimov, University of Southern Denmark.
