Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB1726 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1726, RRID:AB_1238094
- Product name
- MIB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MIB1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta.
A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK.
Itoh M, Kim CH, Palardy G, Oda T, Jiang YJ, Maust D, Yeo SY, Lorick K, Wright GJ, Ariza-McNaughton L, Weissman AM, Lewis J, Chandrasekharappa SC, Chitnis AB
Developmental cell 2003 Jan;4(1):67-82
Developmental cell 2003 Jan;4(1):67-82
A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK.
Jin Y, Blue EK, Dixon S, Shao Z, Gallagher PJ
The Journal of biological chemistry 2002 Dec 6;277(49):46980-6
The Journal of biological chemistry 2002 Dec 6;277(49):46980-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The MIB1 polyclonal antibody (Cat # PAB1726) is used to detect FLAG-tagged MIB1 in 293T cell lysate. Data courtesy of Dr. Olga Ossipova, Mt. Sinai School of Medicine.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells incubated with MIB1 polyclonal antibody (Cat # PAB1726) at a dilution of 1 : 20. Data courtesy of Dr. Vyacheslav Akimov, University of Southern Denmark.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MIB1 polyclonal antibody (Cat # PAB1726) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)