Antibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-450-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL3/MIP-1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL3/MIP-1 alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.03% cross-reactivity with recombinant human (rh) CCL3, recombinant mouse (rm) CCL9/10, and rmCCL4 and less than 0.4% cross-reactivity with rhCCL7.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q5QNW0
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC.
B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation.
Hippocampal T cell infiltration promotes neuroinflammation and cognitive decline in a mouse model of tauopathy.
IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Recruitment and differentiation of conventional dendritic cell precursors in tumors.
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
Selective macrophage suppression during sepsis.
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, Said N
Oncogene 2019 May;38(22):4366-4383
Oncogene 2019 May;38(22):4366-4383
B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation.
Sun W, Meednu N, Rosenberg A, Rangel-Moreno J, Wang V, Glanzman J, Owen T, Zhou X, Zhang H, Boyce BF, Anolik JH, Xing L
Nature communications 2018 Dec 3;9(1):5127
Nature communications 2018 Dec 3;9(1):5127
Hippocampal T cell infiltration promotes neuroinflammation and cognitive decline in a mouse model of tauopathy.
Laurent C, Dorothée G, Hunot S, Martin E, Monnet Y, Duchamp M, Dong Y, Légeron FP, Leboucher A, Burnouf S, Faivre E, Carvalho K, Caillierez R, Zommer N, Demeyer D, Jouy N, Sazdovitch V, Schraen-Maschke S, Delarasse C, Buée L, Blum D
Brain : a journal of neurology 2017 Jan;140(1):184-200
Brain : a journal of neurology 2017 Jan;140(1):184-200
IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.
Thaney VE, O'Neill AM, Hoefer MM, Maung R, Sanchez AB, Kaul M
Scientific reports 2017 Apr 20;7:46514
Scientific reports 2017 Apr 20;7:46514
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
The American journal of pathology 2011 Dec;179(6):2730-9
The American journal of pathology 2011 Dec;179(6):2730-9
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Recruitment and differentiation of conventional dendritic cell precursors in tumors.
Diao J, Zhao J, Winter E, Cattral MS
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1261-7
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1261-7
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
The Journal of experimental medicine 2008 Sep 1;205(9):2075-84
The Journal of experimental medicine 2008 Sep 1;205(9):2075-84
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
European journal of immunology 2006 Aug;36(8):2025-34
European journal of immunology 2006 Aug;36(8):2025-34
Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
Perrin FE, Lacroix S, Avilés-Trigueros M, David S
Brain : a journal of neurology 2005 Apr;128(Pt 4):854-66
Brain : a journal of neurology 2005 Apr;128(Pt 4):854-66
Selective macrophage suppression during sepsis.
Ellaban E, Bolgos G, Remick D
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Cardona AE, Gonzalez PA, Teale JM
Infection and immunity 2003 May;71(5):2634-42
Infection and immunity 2003 May;71(5):2634-42
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Cardona AE, Gonzalez PA, Teale JM
Infection and immunity 2003 May;71(5):2634-42
Infection and immunity 2003 May;71(5):2634-42
Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
Horner AA, Datta SK, Takabayashi K, Belyakov IM, Hayashi T, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA, Richman DD, Raz E
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1584-91
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1584-91
Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
Horner AA, Datta SK, Takabayashi K, Belyakov IM, Hayashi T, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA, Richman DD, Raz E
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1584-91
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1584-91
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse CCL3/MIP-1 alpha by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL3/MIP-1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL3/MIP-1 alpha at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL3/MIP-1 alpha in RAW 264.7 Mouse Cell Line. CCL3/MIP-1 alpha was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse CCL3/MIP-1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL3/MIP-1 alpha in Mouse Thymus. CCL3/MIP-1 alpha was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL3/MIP-1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL3/MIP-1 alpha and Neutralization by Mouse CCL3/MIP-1 alpha Antibody. Recombinant Mouse CCL3/MIP-1 alpha (Catalog # 450-MA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/ MIP-1 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL3/MIP-1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA). The ND50 is typically 0.05-0.2 µg/mL.