Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF-450-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CCL3/MIP-1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL3/MIP-1 alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.03% cross-reactivity with recombinant human (rh) CCL3, recombinant mouse (rm) CCL9/10, and rmCCL4 and less than 0.4% cross-reactivity with rhCCL7.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC.
B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation.
Hippocampal T cell infiltration promotes neuroinflammation and cognitive decline in a mouse model of tauopathy.
IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Recruitment and differentiation of conventional dendritic cell precursors in tumors.
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
Selective macrophage suppression during sepsis.
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, Said N
Oncogene 2019 May;38(22):4366-4383
Oncogene 2019 May;38(22):4366-4383
B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation.
Sun W, Meednu N, Rosenberg A, Rangel-Moreno J, Wang V, Glanzman J, Owen T, Zhou X, Zhang H, Boyce BF, Anolik JH, Xing L
Nature communications 2018 Dec 3;9(1):5127
Nature communications 2018 Dec 3;9(1):5127
Hippocampal T cell infiltration promotes neuroinflammation and cognitive decline in a mouse model of tauopathy.
Laurent C, Dorothée G, Hunot S, Martin E, Monnet Y, Duchamp M, Dong Y, Légeron FP, Leboucher A, Burnouf S, Faivre E, Carvalho K, Caillierez R, Zommer N, Demeyer D, Jouy N, Sazdovitch V, Schraen-Maschke S, Delarasse C, Buée L, Blum D
Brain : a journal of neurology 2017 Jan;140(1):184-200
Brain : a journal of neurology 2017 Jan;140(1):184-200
IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.
Thaney VE, O'Neill AM, Hoefer MM, Maung R, Sanchez AB, Kaul M
Scientific reports 2017 Apr 20;7:46514
Scientific reports 2017 Apr 20;7:46514
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
The American journal of pathology 2011 Dec;179(6):2730-9
The American journal of pathology 2011 Dec;179(6):2730-9
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Recruitment and differentiation of conventional dendritic cell precursors in tumors.
Diao J, Zhao J, Winter E, Cattral MS
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1261-7
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1261-7
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
The Journal of experimental medicine 2008 Sep 1;205(9):2075-84
The Journal of experimental medicine 2008 Sep 1;205(9):2075-84
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
European journal of immunology 2006 Aug;36(8):2025-34
European journal of immunology 2006 Aug;36(8):2025-34
Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
Perrin FE, Lacroix S, Avilés-Trigueros M, David S
Brain : a journal of neurology 2005 Apr;128(Pt 4):854-66
Brain : a journal of neurology 2005 Apr;128(Pt 4):854-66
Selective macrophage suppression during sepsis.
Ellaban E, Bolgos G, Remick D
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Cardona AE, Gonzalez PA, Teale JM
Infection and immunity 2003 May;71(5):2634-42
Infection and immunity 2003 May;71(5):2634-42
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Cardona AE, Gonzalez PA, Teale JM
Infection and immunity 2003 May;71(5):2634-42
Infection and immunity 2003 May;71(5):2634-42
Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
Horner AA, Datta SK, Takabayashi K, Belyakov IM, Hayashi T, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA, Richman DD, Raz E
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1584-91
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1584-91
Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
Horner AA, Datta SK, Takabayashi K, Belyakov IM, Hayashi T, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA, Richman DD, Raz E
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1584-91
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1584-91
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse CCL3/MIP-1a by Western Blot. Detection of Mouse CCL3/MIP-1a by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CCL3/MIP-1a in Mouse Thymus. CCL3/MIP-1a in Mouse Thymus.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL3/MIP-1a and Neutralization by Mouse CCL3/MIP-1a Antibody. Chemotaxis Induced by CCL3/MIP-1a and Neutralization by Mouse CCL3/MIP-1a Antibody.
