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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-270-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL3/MIP-1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CCL3/MIP-1 alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) MIP-1 beta , rhMIP-1 delta , rhMIP-3 alpha , rhMIP-3 beta , and recombinant mouse (rm) MIP-1 alpha is observed. Neutralizes the biological activity of recombinant human CCL3/MIP-1 alpha . In the chemotaxis assay this antibody will also partially neutralize the biological activity of rhMIP-1 beta at a 20 fold higher IgG concentration. It will not neutralize the biological activity of rmMIP-1 beta or rmMIP-1 alpha .
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P10147- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Increased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers.
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
MIP-1alpha (CCL3) is a downstream target of FGFR3 and RAS-MAPK signaling in multiple myeloma.
Gene copy number regulates the production of the human chemokine CCL3-L1.
Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
Walker WE, Kurscheid S, Joshi S, Lopez CA, Goh G, Choi M, Barakat L, Francis J, Fisher A, Kozal M, Zapata H, Shaw A, Lifton R, Sutton RE, Fikrig E
Journal of virology 2015 May;89(10):5502-14
Journal of virology 2015 May;89(10):5502-14
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Pfleger C, Schloot N, ter Veld F
Journal of immunological methods 2008 Jan 1;329(1-2):214-8
Journal of immunological methods 2008 Jan 1;329(1-2):214-8
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clinical chemistry 2007 Nov;53(11):2010-2
Clinical chemistry 2007 Nov;53(11):2010-2
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clinical chemistry 2007 Nov;53(11):2010-2
Clinical chemistry 2007 Nov;53(11):2010-2
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS (London, England) 2007 Jun 19;21(10):1263-72
AIDS (London, England) 2007 Jun 19;21(10):1263-72
MIP-1alpha (CCL3) is a downstream target of FGFR3 and RAS-MAPK signaling in multiple myeloma.
Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio JO, Bergsagel PL, Stewart AK
Blood 2006 Nov 15;108(10):3465-71
Blood 2006 Nov 15;108(10):3465-71
Gene copy number regulates the production of the human chemokine CCL3-L1.
Townson JR, Barcellos LF, Nibbs RJ
European journal of immunology 2002 Oct;32(10):3016-26
European journal of immunology 2002 Oct;32(10):3016-26
Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
Binninger-Schinzel D, Norley S, Adler HS, Oberg HH, Kurth R
Virology 2002 Mar 1;294(1):209-21
Virology 2002 Mar 1;294(1):209-21
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL3/MIP-1 alpha in Human Tonsil. CCL3/MIP-1 alpha was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CCL3/MIP-1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-270-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL3/MIP-1 alpha and Neutral-ization by Human CCL3/ MIP-1 alpha Antibody. Recombinant Human CCL3/MIP-1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP-1 alpha (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP-1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-270-NA). The ND50 is typically 2-8 µg/mL.
