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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 627602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#627602, RRID:AB_2086967
- Product name
- Purified anti-PARC (H7AP1)
- Antibody type
- Monoclonal
- Antigen
- Human PARC peptide
- Description
- The PARC protein, also known as p53-associated parkin-like cytoplasmic protein and UbcH7 associated protein 1, is a large (>250 kD) ubiquitously expressed cytoplasmic protein that contains a cullin domain and signature RING-IBR (in-between ring finger domain)-RING domains. PARC is thought to serve as a cytoplasmic anchor in p53-associated complexes thought to be critical in controlling p53 subcellular localization and function. These large complexes that contain PARC, p53 and other associated proteins are approximately 1 MD in non-stressed cells. The PARC protein has been shown to interact with a number of proteins including p53, UBCH8, ubiquitin conjugating enzyme E2G2, and ubiquitin conjugating enzyme E2L3. The P069 monoclonal antibody recognizes human PARC and has been shown to be useful for Western blotting.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4° C.
Submitted references Inhibition of Thr-55 phosphorylation restores p53 nuclear localization and sensitizes cancer cells to DNA damage.
Cai X, Liu X
Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 4;105(44):16958-63
Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 4;105(44):16958-63
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- HepG2 cell extract (left lane) or NIH 3T3 cell extract (right lane) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PARC antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. In all human cell lines tested, this antibody recognizes a non-specific 66 kD protein in addition to PARC.
- Conjugate
- Unconjugated
