PA5-120538

antibody from Invitrogen Antibodies

Targeting: ZCCHC3 C20orf99, dJ1103G7.7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120538

Provider

Invitrogen Antibodies

Product name

ZCCHC3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: LO2, mouse liver.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.35 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ZCCHC3 in various cell lines using a ZCCHC3 Polyclonal antibody (Product # PA5-120538) at a dilution of 1:3,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of ZCCHC3 in L929 cells using a ZCCHC3 Polyclonal antibody (Product # PA5-120538). Blue: DAPI for nuclear staining.