sc-7289

antibody from Santa Cruz Biotechnology

Targeting: PTPN6 HCP, HCPH, PTP-1C, SHP-1, SHP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-7289

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-7289, RRID:AB_628251

Product name

Anti-PTPN6

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Mouse

References

Submitted references

Double knockouts reveal that protein tyrosine phosphatase 1B is a physiological target of calpain-1 in platelets.

Kuchay SM, Kim N, Grunz EA, Fay WP, Chishti AH
Molecular and cellular biology 2007 Sep;27(17):6038-52

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Silencing of SH-PTP2 defines a crucial role in the inactivation of epidermal growth factor receptor by 5-aminosalicylic acid in colon cancer cells

G Monteleone, L Franchi, D Fina, R Caruso, P Vavassori, I Monteleone, E Calabrese, G C Naccari, S Bellinvia, R Testi, F Pallone
Cell Death and Differentiation 2005 Aug;13(2):202-211

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 70

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.