AP2811C

antibody from Abgent

Targeting: PTPN6 HCP, HCPH, PTP-1C, SHP-1, SHP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2811C

Provider

Abgent

Proper citation

Abgent Cat#AP2811c, RRID:AB_1554058

Product name

PTPN6 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PTPN6 Antibody (Center) (Cat.#AP2811c) in HL-60 cell line lysates (35ug/lane).PTPN6 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PTPN6 (arrow) using rabbit polyclonal PTPN6 Antibody (Center) (Cat. #AP2811c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2) (Origene Technologies).(2ug/ml)

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Immunofluorescent analysis of U251 cells, using PTPN6 Antibody (Center) (Cat. #AP2811c). AP2811c was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue)."

Primary Ab dilution

1:100

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50