A04133-2

antibody from Boster Biological Technology

Targeting: MARCKS 80K-L, MACS, PKCSL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

A04133-2

Provider

Boster Biological Technology

Product name

Anti-MARCKS (Ab-158) Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for P29966 detection. Tested positive for WB IF in Hu Ms Rt

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100ul

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store at -20°C for long term preservation (recommended) . Store at 4°C for short term use.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of MARCKS using anti-MARCKS antibody (A04133-2).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCKS antigen affinity purified polyclonal antibody (Catalog # A04133-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MARCKS.

Additional image