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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB110-58875 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-58875, RRID:AB_877699
- Product name
- Rabbit Polyclonal MARCKS Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: MARCKS Antibody [NB110-58875] - Analysis of cell line lysates probed simultaneously with rabbit pAb to MARCKS, dilution 1:1000, in green and mouse mAb to GAPDH, dilution 1:5000 in red: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, [7] C6 cells. The strong band at ~80kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells [5], but no reactivity is seen on rodent cells, [6] and [7]. GAPDH antibody used as a loading control revealing a single band at ~37kDa in all preparations.
